Figure 2
NIS mRNA regulation in different thyroid statuses in NIS abundant tissues. A. Expression of NIS mRNA through Northern blot analysis. Total RNA (8 μg for thyroid and 15 μg for stomach) from animals under different thyroid statuses was loaded on 0.8% agarose gel, transferred to the Nylon membrane and hybridized to a 32P-labeled NIS specific DNA probe (upper lane). The same membrane used in the upper lane was rehybridized to 18S probe to validate the consistency of loaded amount of RNA. B. Expression of NIS mRNA through quantitative real-time RTPCR (qPCR). 25 ng purified RNA-equivalent cDNA was amplified in one reaction. Expression of each target gene (thyroid, left panel; stomach, right panel) was measured in duplicate and was normalized relative to 18S ribosomal RNA expression. Results are shown as mean ± SEM. * P < 0.05 compared with control (unpaired t-test). Control, C; hypothyroid, L; and hyperthyroid, H.