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Table 3 Amplification conditions for RET/PTC1 and RET/PTC3 rearrangements using real-time PCR.

From: Assessment of RET/PTC1 and RET/PTC3 rearrangements in fine-needle aspiration biopsy specimens collected from patients with Hashimoto's thyroiditis

Times and temperatures

  

Each of 45 cycles

Initial setup

Denaturation

Annealing/Elongation

HOLD

HOLD

CYCLE

UNG activation 2 min, 50°C

10 min, 95°C

15 s, 95°C

1 min, 60°C

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Thyroid Research

ISSN: 1756-6614