A study to evaluate association of nuclear grooving in benign thyroid lesions with RET/PTC1 and RET/PTC3 gene translocation

Table 1 Shows RET/PTC1, RET/PTC3, and ACTB primer–probe sequences. Rhoden KJ. [13]

RET/PTC1	Forward primer	GAACCGCGACCTGCGCAAA
RET/PTC1	Reverse Primer	CAAGTTCTTCCGAGGGAATTCC
RET/PTC1	Probe	6 FAM- CAA GCG TAA CCA TCG AGG ATC CAA AGT-TAMRA
RET/PTC3	Forward primer	CCCCAGGACTGGCTTACCC
RET/PTC3	Reverse Primer	CAAGTTCTTCCGAGGGAATTCC
RET/PTC3	Probe	6FAM-AAA GCA GAC CTT GGA GAA CAG TCA GGA GG-TAMRA
ACTB	Forward primer	AGC CTC GCC TTT GCC GA
ACTB	Reverse Primer	CTG GTG CCT GGG GCG
ACTB	Probe	VIC- CCG GCT TCG CGG GCG AC—TAMRA

ISSN: 1756-6614