A study to evaluate association of nuclear grooving in benign thyroid lesions with RET/PTC1 and RET/PTC3 gene translocation

Table 2 Shows PCR conditions for the Taqman gene expression assay

Settings	Step 1	Step 2	Step 3
Stage	Reverse transcription	denaturation	PCR (denaturation)	PCR (anneal/extend)
Temperature	45⁰	95⁰	95⁰	60⁰
time	10 min	10 min	15 s	45 s

ISSN: 1756-6614