Table 3 EABA in thyroid tissue according to published studies
From: Endogenous avidin biotin activity (EABA) in thyroid pathology: immunohistochemical study
Antigen retrieval method | Type of thyroid lesion | Observation | |
|---|---|---|---|
Bussolati et al. (1997) [9] | Autoclaving; microwave | OTC | Expression ++ in 5/5 |
PTC | Expression + in 3/5 | ||
Kashima K et al. (1997) [6] | Trypsin pretreatment; Autoclaving | Various thyroid lesions | Expression + in 93/208 |
PTC and FTC | Higher expression | ||
ATC, squamous cell carcinoma; poorly differentiated carcinoma | Lowest expression | ||
Zhou X et al. (2002) [4] | Microwaving in EGTA buffer pH 9.0; 8.0; 6.0 | C cells, PTC, OTC | EABA was easier revealed by higher pH value buffer (EGTA pH 9.0) than that with lower pH value (EDTA pH 8.0 and citrate pH 6.0) |
Herrmann ME et al. (2002) [13] | Microwaving in 0.1 Tris buffer pH 7.4 | PTC, reactive oxyphilic cells | identified with biotin detection system when the primary antibody was omitted |
Mutsomoto F et al. (2006) [12] | Microwaving in citric acid (pH 6.0) for 10 minutes at 100°C | oxyphilic cells | Elimination of nonspecific staining due to endogenous avidin-biotin activity by using En-Vision+ system HRP-labeled polymer |