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Table 3 EABA in thyroid tissue according to published studies

From: Endogenous avidin biotin activity (EABA) in thyroid pathology: immunohistochemical study

  Antigen retrieval method Type of thyroid lesion Observation
Bussolati et al. (1997) [9] Autoclaving; microwave OTC Expression ++ in 5/5
   PTC Expression + in 3/5
Kashima K et al. (1997) [6] Trypsin pretreatment; Autoclaving Various thyroid lesions Expression + in 93/208
   PTC and FTC Higher expression
   ATC, squamous cell carcinoma; poorly differentiated carcinoma Lowest expression
Zhou X et al. (2002) [4] Microwaving in EGTA buffer pH 9.0; 8.0; 6.0 C cells, PTC, OTC EABA was easier revealed by higher pH value buffer (EGTA pH 9.0) than that with lower pH value (EDTA pH 8.0 and citrate pH 6.0)
Herrmann ME et al. (2002) [13] Microwaving in 0.1 Tris buffer pH 7.4 PTC, reactive oxyphilic cells identified with biotin detection system when the primary antibody was omitted
Mutsomoto F et al. (2006) [12] Microwaving in citric acid (pH 6.0) for 10 minutes at 100°C oxyphilic cells Elimination of nonspecific staining due to endogenous avidin-biotin activity by using En-Vision+ system HRP-labeled polymer