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Table 3 Real-time PCR conditions for PIN1 gene amplification.

From: Increased expression of PIN1 gene in papillary thyroid carcinoma

Times and temperatures

Initial setup

Each of 40 cycles

  

Denaturation

Annealing/Elongation

HOLD

HOLD

CYCLE

UNG activation 2 min, 50°C

10 min, 95°C

15 s, 95°C

1 min, 60°C

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Thyroid Research

ISSN: 1756-6614