Skip to main content

Table 3 Conditions of restriction analysis

From: Association between genetic mutations and the development of autoimmune thyroiditis in patients with chronic hepatitis C treated with interferon alpha

Gene

Polymorphism

Restriction enzyme

Restriction fragments [bp]

CTLA4

rs 231775 A/G

Sat I [10 U, 37°C, 12 h]

allele A: 99 + 63

allele G: 74 + 25 + 63

rs5742909

Mse I [5 U, 65°C , 12 h]

allele C: 215

allele T: 124 + 91

PTPN22

rs2476601 C/T

Rsa I [5 U, 37°C, 12 h]

allele C: 228 + 74 + 46 + 44

allele T: 272 + 74 + 46