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Fig. 4 | Thyroid Research

Fig. 4

From: Alginate-based 3D cell culture technique to evaluate the half-maximal inhibitory concentration: an in vitro model of anticancer drug study for anaplastic thyroid carcinoma

Fig. 4

Inhibitory effects of BI-847325 on viability of ATC cell lines. C643 and SW1736 cell lines were treated with the indicated concentrations of BI-847325 in (A) 2D and (B) 3D cell culture systems for 24–72 h, followed by MTT assay to evaluate cell viability. IC50 values of BI-847325 for each cell line in two cell culture systems were calculated according to the Reed-Muench methods. The viability of the cells treated by different concentration of drug was normalized to that of untreated ones which were exposed to DMSO with the concentration equal to what existed in drug solutions. This normalization process was performed for each time of assay (i.e. 24, 48, and 72 h) separately. For each time point, the viabilities of the cells treated with different concentration of BI-847325 were compared and statistically analyzed via One-way ANOVA and Turkey’s post-test method. The significant differences comparing the control (i.e., untreated) cells are represented by star(s) as follows: *p < .05, **p < .01, ***p < .001, ****p < .0001)

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Thyroid Research

ISSN: 1756-6614