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Table 3 Primers sequences applied for quantitative Real-Time PCR

From: Expression of somatostatin receptor subtypes in human thyroid tumors: the immunohistochemical and molecular biology (RT-PCR) investigation

Name

Sequence

Size of PCR product, bp

GenBank accession no.

SSTR 2A (forward)

SSTR 2A (reverse)

5'ATGCCAAGATGAAGACCATCAC 3'

5'TGAACTGATTGATGCCATCCA 3'

171 [19]

NM 001050

SSTR 3 (forward)

SSTR 3 (reverse)

5'CTGGGTAACTCGCTTGGTCATCTA 3'

5'AGCGCCAGGTTGAGGATGTA 3'

86 [19]

NM 001051

SSTR 1 (forward)

SSTR 1 (reverse)

5'TATCTGCCTGTGCTACGTGC 3'

5'GATGACCGACAGCTGACTCA 3'

217 [20]

NM 001049

SSTR 4 (forward)

SSTR 4 (reverse)

5'ATCTTCGCAGACACCAGACC 3'

5'ATCAAGGCTGGTCACGACGA 3'

321 [19]

NM 001052

SSTR 5 (forward)

SSTR 5 (reverse)

5'GTGACAACAGGACGCTGGT 3'

5'TGGTGACGGTCTTCATCTTG 3'

156 [20]

NM 001053

HPRT (forward)

HPRT (reverse)

5'TGCTTTCCTTGGTCAGGCAGTAT 3'

5'TCAAATCCAACAAAGTCTGGCTTATATC3'

109 [19]

NM 000194

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Thyroid Research

ISSN: 1756-6614