Figure 4

Oxidative damage to mitochondrial DNA in porcine thyroid. mtDNA was incubated in the presence of both substrates used together, namely in the presence of 0.5 mM H₂O₂ plus FeSO₄ [300, 150, 30, 15, 3.0, 1.5 μM]. Data are expressed as the ratio 8-oxodG/dGx10⁵. Data are from three independent experiments. Values are expressed as mean ± SE (error bars). ^ap = 0.05 vs. control (in the absence of both Fe²⁺ and H₂O₂), ^bp = 0.05 vs. 300 μM, ^cp = 0.05 vs. 150 μM.